To calculate the amount of NADPH required to make a 2 millimolar solution with a volume of 100 ml, you can use the formula:
Amount (in grams)=Concentration (in mol/L)×Volume (in L)×Molecular weight (in g/mol)
First, let's convert the concentration from millimolar (mM) to molar (M):
2mM=2×10−3M
The molecular weight of NADPH is approximately 744.42 g/mol.
Now, we can plug these values into the formula:
Amount (in grams)=(2×10−3M)×(100×10−3L)×744.42g/mol
Amount (in grams)=2×10−5×744.42g
Amount (in grams)=0.0148884g
Now, let's convert this amount to milligrams:
0.0148884g×1000mg/g=14.8884mg
So, approximately 14.8884 milligrams of NADPH will be required to make a 2 millimolar solution with a volume of 100 ml.
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